Microsart® AMP Mycoplasma Kit
Reduce your testing time from weeks to 3 hours with the Microsart® AMP Mycoplasma. With the ability to be very flexible in sample volume (up to 18 ml) and sample preparation, the kit is specifically designed for: the control of raw materials, for in-process control, and for lot release testing of finished products.
Rapid methods for the detection of mycoplasma contamination are essential for improving monitoring of the production process of biologicals. The Microsart® AMP Mycoplasma is the first member of a qPCR product line of kits for mycoplasma detection. The Microsart® AMP Mycoplasma can be easily implemented because no specific hardware is needed. The kit can be used with any type of real-time PCR cycler that is able to detect the fluorescence dyes FAM™ and ROX™. The total testing procedure takes only 3 hours. The Microsart® AMP Mycoplasma is effectively validated according to chapter EP 2.6.7 in combination with EP 2.6.21 for sensitivity, specificity and robustness. Carefully selected primer/probe combinations are highly specific for a region within the 16S rRNA gene for more than 110 Mycoplasma species. Due to the optional prior enrichment step using the Vivaspin® 20 or Vivaspin® 6 the starting volume for the DNA extraction is flexible. You have the option to analyze a testing volume from 200 µl up to 18 ml which ensures highest sensitivity. The kit contains all essential components in a function tested ready-to-use master mix, including the polymerase.
CUSTOMER BENEFITS:
- Minimizes testing time from weeks down to hours
- Microsart® AMP Mycoplasma is validated according to EP 2.6.7
- Convenient and simple use
- For use with all common qPCR cyclers able to detect FAM ™- and ROX™-labeled probes
- The product fulfills all the different demands in regard to sensitivity and security